001). The DAI score of all drug groups were decrease by dose dependent The pathohistological severity score of colon(The degrees of colon inflammation, pathological depth and crypt destruction) were significantly lower in control group than those in model group and all drug groups (P < 0.05, 0.01); In the same dosage, The pathohistological severity score of colon were decrease by dose dependent; and LY294002 sinomenine groups were significantly higher than that of sinomenine microsphere
groups corresponding to the same doses groups (P < 0.05, 0.01). Conclusion: The sinomenine and the sinomenine chitosan microspheres could relieve the symptoms of inflammatory and pathological damage in experimental colitis mice. The effect of sinomenine microspheres on reduce symptoms, inflammation and pathological damage is better than that of Sinomenine in same dose. selleckchem Key Word(s): 1. Sinomenine; 2. Colitis; 3. Microspheres; 4. RCT; Presenting Author: BING XIA Additional Authors: QIAO YU, SIYING ZHU, RUI ZHOU, FENGMING YI, YUNTAO BING, SHA HUANG, ZIXI WANG, CHUNYU WANG Corresponding Author: BING XIA Affiliations: Zhongnan
Hospital Objective: Sinomenine, a pure alkaloid isolated from the Chinese medical root of Sinomenium acutum, has been demonstrated to have anti-inflammatory and immunosuppressive effects. Micro-RNAs (miRNAs) are gradually recognized as critical mediators of disease pathogenesis via coordinated regulation of molecular effector pathways. Methods: After 2, 4, 6-Trinitrobenzenesulfonic acid (TNBS) induced colitis in mice colitis was induced by colonic instillation of 5% (w/v) TNBS, sinomenine at a dose of 100 or 200 mg/kg was orally administered once daily for 7 days in mouse models. We evaluated body weight, survival rate, diarrhea score, histological score and myeloperoxidase (MPO) activity. The expression levels of miR-155, c-Maf, 上海皓元 TNF-α and IFN-γ were respectively determined by quantitative real-time polymerase chain reaction and immunohistochemistry.
Results: Sinomenine (100 or 200 mg/kg)-treated mice with TNBS-induced colitis significantly improved in weight, survival rate, diarrhea score, histological score and MPO activity with respect to untreated mice. Both dosages of sinomenine significantly decreased the mRNA and protein expressions of c-Maf, TNF-α and IFN-γ which are elevated in TNBS. Furthermore, sinomenine at a dose of 200 mg/kg could significantly decrease the level of miR-155 by 71% (p = 0.025) compared with untreated TNBS-induced mice. Conclusion: Our study evaluate the effects and potential mechanisms of sinomenine in anti-inflammatory response via microRNA-155 in TNBS induced colitis in mice.Our findings suggest that sinomenine has anti-inflammatory effect on TNBS induced colitis by down-regulating the levels of miR-155 and related inflammatory cytokines. Key Word(s): 1. IBD; 2. TNBS; 3. Sinomenine; 4.