Overall, 51% of

InC3 participants were IL28B CC positive, and in the subpopulation studied 52% (139/267) were CC positive. Among 137 with SI, 56% were IL28B CC compared to 46% of asymptomatic patients in models adjusting for age and sex (Adjusted odds ratio [AOR] 0.7, 95% CI: 0.4, 1.1). 64% of patients with jaundice were IL28B CC genotype compared to 42% of those without jaundice (AOR 0.3, 95% CI:0.1, 0.9).69% of patients with elevated ALT were IL28B CC positive compared to 43% of those without elevated ALT (AOR 0.3, 95% CI: 0.2,0.6). Conclusions: IL28B CC genotype is associated selleck chemicals with elevated ALT and jaundice during acute HCV infection among patients with seroconversion illness. The association between symptoms of acute HCV and clearance reported in many studies may be related to IL28B status. Disclosures: Barbara H. McGovern – Employment: AbbVie Jason Grebely – Advisory Committees or Review Panels: Merck, Merck, Merck, Merck; Grant/Research Support: Merck, Merck, Merck, Merck Arthur Y. Kim – Consulting: Abbvie Pharmaceuticals, Gilead Pharmaceuticals; Grant/Research Support: Bristol-Myers Squibb Maria Prins – Speaking and Teaching: msd, roche Gregory J. Dore Sirolimus ic50 – Board Membership: Bristol-Myers Squibb, Roche, Gilead, Merck, Janssen, Abbvie; Grant/Research Support: Janssen, Bristol-Myers Squibb, Vertex, Roche, Gilead, Merck, Abbvie; Speaking

and Teaching: Roche, Merck, Janssen The following people have nothing to disclose: Kimberly Page, Jennifer Evans, Meghan D. Morris, Andrea Cox, Thomas M. Rice,

Rachel Sacks-Davis, Margaret Hellard, Julie Bruneau, Naglaa Shoukry, Lisa Maher, Andrew R. Lloyd Background and aims: NK cells display anti-fibrotic activity via killing of activated hepatic stellate cells (HSC). NK cell function is regulated by cross-talk with other immunocompetent cells as well as soluble factors. Recently, we demonstrated that regulatory T cells in hepatitis C produce high amounts of IL-8 and induce up-regulation of profibrogenic learn more markers in human primary HSC (Langhans et al., J Hepatology 2013). Here, we analyzed in vitro whether stimulation of human primary HSC with profibrogenic cytokines results in altered activation of NK cells. Methods: Human primary HSC (ScienCell Research Laboratories) were pre-cultured in vitro in the absence or presence of recombinant IL-8 or IL-10 (0–100 ng/ml each). HSC were co-cultured with purified peripheral NK cells from healthy donors at 1:1 ratio. After 24 hours activation of NK cells was determined by flow cytometric analysis of NK cell degranula-tion (CD107a expression). Results: Compared to untreated HSC, CD107a expression of CD56brightCD16negative NK cells was significantly reduced in co-cultures using IL-8 pre-treated HSC (p>0,005). Reduced NK cell degranulation was dose dependent.