Dir Gerung start new analysis Vorinostat MK-0683 and the direct injection of samples in the order of 19 s each dwell time 150 ms for the IMA measurement has 15 points per peak, the most reliable, precious metals, for accurate and pr Precise quantification. 3.3. To validate the linearity of t of the method, LOD, LOQ, imprecision, recovery, analysis, and accuracy were checked determined. The method provides more linear dependence Dependencies entire series with correlation coefficient r 0.994. The limits of quantification than 6 ng / ml for the analytes in the MRM mode measured are well below the clinically relevant range of concentrations encountered in patients. In the case of DAS MRM3 LQ mode was five times lower, corresponding to DNA-PK inhibition the demand for green Erer sensitivity in response to lower plasma levels. Inaccuracies day and intra and inter-information in coefficient of variation and bias groups are summarized in Table 3. Applied to blood samples from IQC and EQC, the method excellent bias and standard deviation. TKI stability t under various conditions has been described in previously published studies. All analytes were stored in plasma for at least five months with a maximum loss of 10% of the nominal concentration. The removal of ions using plasma samples were added at low concentrations, medium and high TKI in six repetitions was significant h Forth compared to liquid chromatographic method according to the ionization of the removal of many substances theion in the source may need during the time, the same analysis. Ion losses were 66.5 to 74.1%, 77.8 to 86,9%, 54.0 to 60.0% and 65.3 to 84.0% for the IMA, NIL, DAS, and LAP or .. Thank you to the use of deuterated internal standards, matrix effects were eliminated. The analysis of direct injection and LC methods were compared by Bland Altman and regression analysis. The contr The quality, and patient samples were analyzed by paired test. Bland Altman showed a mean difference of 0.00 ng / ml and a standard deviation of 39.22, 21.73, 113.24 and 8.36 ng / mL for IMA, NIL, and THE LAP respectively. Regression analysis gave a linear MK-8669 correlation with a slope of 1.023, 1.005, 0.995, 1.029 intercept 0158, 0395, 2881, 5773, and correlation coefficient of 0.997, 0.999, 0.994 and 0.998 for IMA, NIL, and THE LAP respectively. 4th Conclusions targeted therapy with IMA, NIL, and THE LAP is based on inhibition of protein tyrosine kinases, an emerging concept in therapeutic strategies. monitoring plasma concentrations of TKI is an essential tool for assessing response to treatment and management of CML patients with or breast cancer. The aim of our study was to develop a high throughput method for determination of plasma concentrations of TKI. Compared to before Ver published shall process using HPLC MS / MS, our method is a brief analysis of 55 s or 19 in the multiple injection parameters for the exclusion of the separation step and sample preparation for deproteinization with organic Solvent is relatively simple. Is therefore the determination of several important cancer drugs in plasma by isotope dilution mass spectrometry, direct injection method rapid, sensitive, selective power, and h Ago, ben Requires a more limited amount of plasma sample, and a significantly new.