Although subjects were experienced athletes and the exercises used in the fatigue protocol were all familiar to them, the physical stress was strong enough to generate the response observed. Lactate concentration decreased significantly during warm up on FG on both days (PRE SETS compared to FATIGUE). The warm up specific exercises had its own particular purpose for the athletes but it might have worked as an active recovery process regarding the metabolic response to fatigue

protocol, as described by [19]. Lactate concentration was not different when compared to CG on PRE SETS (WATER DAY–lactate 3.94 ± 3.23 mmol/L FG and 2.2 ± 0.81 mmol/L CG p = 0.27) (CARBOHYDRATE DAY–lactate 5.2 ± 1.5 mmol/L FG and 4.75 ± 2.83 mmol/L CG p = 0.73) probably because of the warm up exercises that might have helped to clear the lactate. Although the FG athletes might have recovered their lactate concentration #TPX-0005 datasheet randurls[1|1|,|CHEM1|]# levels, they showed some visual signs of fatigue and they reported to us as feeling fatigued, although we can’t consider that as a measured variable. Lactate did not show any differences on both points PRE SETS and POST SETS on WATER DAY (2.2 ± 0.8 mmol/L PRE SETS and 2.3 ± 1.4 mmol/L POST SETS for CG p = 0.88 and 3.94 ± 3.23 mmol/L PRE SETS and 3.68 ± 1.87 mmol/L POST SETS for FG p = 0.91), probably because exercise intensity

was constant during the set. This data corroborates the hypothesis OSI-744 mouse that although the balance beam is one of the most difficult exercises in gymnastics, it is not mainly physically demanding, but it also requires a lot of concentration in order to perform it properly [6]. On CARBOHYDRATE DAY, lactate concentration didn’t change on PRE SETS and POST SETS to CG but was significant lower on POST SETS when compared to PRE SETS to FG (4.75 ± 2.83 mmol/L PRE SETS and 3.30 ± 1.32 mmol/L

POST SETS for CG p = 0.22; 5.2 ± 1.5 mmol/L PRE SETS and 3.7 ± 1.2 mmol/L POST SETS for FG p = 0.03). Lactate values were lower on post sets to FG as a consequence of the stronger removal that was elicited by the higher lactate concentration produced by the fatigue circuit. Lactate data can be observed on Figure 1. Figure 1 Lactate (mmol/L) data to CG and FG on both days. * p < 0.05 Comparing RANTES lactate on FATIGUE with RESTfor the FG group on both days. # p < 0.05 comparing lactate from POST SETS to PRE SETS on both days. On WATER DAY, glucose concentration did not change at any moment, except for the FG on FATIGUE, which showed a trend to a higher glucose concentration compared to rest (WATER DAY–97.2 ± 16.72 mg/dl FG REST; 118 ± 39.1 mg/dl FG FATIGUE p = 0.12) this glucose increase happened due to the high intensity of the fatigue protocol and the consequent hormonal responses to the stress stimulus, as promoted by the HPA axis activation [18].