Furthermore, TPGS effectively inhibited the growth of human

lung BTK inhibitor carcinoma cells both in vitro and in vivo[52]. The superior antitumor activity of TPGS was associated with its increasing ability to induce apoptosis [52–54]. Synergistic anticancer effects could be obtained by the use of combinations of TPGS in the presence of other anticancer agents [53]. Methods Materials TPGS, glycolide (1,4-dioxane-2,5-dione), and stannous octoate were obtained from Sigma-Aldrich (St. Louis, MO, USA). Poly(vinyl alcohol) (PVA; 80% hydrolyzed), branched polyethyleneimine (MW ~ 25,000), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were also DMXAA purchased from Sigma-Aldrich. ε-CL was from Acros Organics (Geel, Belgium). 4′,6-Diamidino-2-phenylindole dihydrocloride (DAPI) was obtained from VECTOR (Burlingame, CA, USA). Plasmid vectors pShuttle2, pIRES2-EGFP, and pDsRED-E1 were acquired from

Invitrogen-Gibco (Carlsbad, CA, USA). MRT67307 in vitro Dulbecco’s modified Eagles’ medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, and Dulbecco’s phosphate-buffered saline (DPBS) were also from Invitrogen. All other chemicals and solvents used were of the highest quality commercially available. Synthesis and characterization of TPGS-b-(PCL-ran-PGA) diblock copolymer TPGS-b-(PCL-ran-PGA) diblock copolymers were synthesized via ring opening copolymerization (ROP) of ε-CL, glycolide, and TPGS as described previously [41]. Briefly, weighted amounts of ε-CL, glycolide, and TPGS and one drop of stannous octoate were added into a dried glass ampoule. The ampoule was connected to a vacuum Carnitine palmitoyltransferase II line, evacuated, sealed off, and placed in oil bath at 160°C. A slow and progressive viscosity increase

of the bulk homogeneous mixture was always observed during the polymerization, and the copolymers were produced in over 3 h. After cooling to room temperature, the ampoule was opened, and the resulting copolymers were dissolved in dichloromethane (DCM) and then precipitated in excess cold methanol to remove unreacted TPGS and monomers. The final product was collected by filtration and dried at 45°C under vacuum. Fourier transform infrared spectroscopy (FT-IR) (Nicolet Instrument Co., Madison, WI, USA) was employed to investigate the chemical structure of TPGS-b-(PCL-ran-PGA) copolymer. Briefly, the samples for FT-IR analysis were prepared by grinding 99% potassium bromide (KBr) with 1% copolymer and then pressing the mixture into a transparent disk in an evacuable die at sufficiently high pressure. The structure, number-averaged molecular weight (Mn) of the copolymer, and copolymer composition were determined by proton nuclear magnetic resonance (1H NMR) in CDCl3 at 300 Hz (Bruker ACF300, Madison, WI, USA). The weight-averaged molecular weight and molecular weight distribution were determined by gel permeation chromatography (Waters, Milford, PA, USA).