The visual results of the macrobroth dilution standard method is

The visual results of the macrobroth dilution standard method is shown on the left (A and D), along with the time course results of the

ETGA (B and E) and gsPCR (C and F) AST analyses, plotting Ct versus time. Vertical, dashed lines indicate when aliquots were removed for analysis. Since Ct values are inversely related to signal selleckchem strength, the y-axes are inverted to visually demonstrate a rise in signal over time. Figure 4 E. coli selleck compound against ciprofloxacin and tetracycline AST results. The visual results of the macrobroth dilution standard method is shown on the left (A and D), along with the time course results of the ETGA (B and E) and gsPCR (C and F) AST analyses, plotting Ct versus time. Vertical, dashed lines indicate when aliquots were removed for analysis. Since Ct values are inversely related to signal strength, the y-axes are inverted to visually

demonstrate a rise in signal over time. Table 1 Comparison of minimum inhibitory concentration results for MSSA, MRSA and E. coli strains S. aureus ATCC 29213         Bacteria from purified cultures Belinostat supplier Bacteria harvested from a positive blood culture bottle Drug Phenotype CLSI MIC interpretation Macrobroth MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation         4 hr 6 hr 22 hr 4 hr 6 hr 22 hr 4 hr 6 hr 4 hr 6 hr Oxacillin Susceptible S ≤ 2 R ≥ 4 0.25 S 1 S 0.5 S 0.5 S 0.5 S 1 S 1 S 0.25 S 0.25 S 0.25 S 0.25 S Vancomycin Susceptible S ≤ 2 I = 4-8 R ≥ 16 < 0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.125 S <0.125 S <0.125 S <0.125 S S. aureus NRS241         Bacteria from purified cultures Bacteria

harvested from a positive blood culture bottle Drug Phenotype CLSI MIC interpretation Macrobroth MIC and interpretation ETGA MIC and interpretation Ribose-5-phosphate isomerase gsPCR MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation         4 hr 6 hr 22 hr 4 hr 6 hr 22 hr 4 hr 6 hr 4 hr 6 hr Oxacillin Resistant S ≤ 2 R ≥ 4 16 R 8 R 16 R > 16 R N/Aa 16 R >16 R 8 R 16 R 8 R 2 Sc (VME) Vancomycin Susceptible S ≤ 2 I = 4-8 R ≥ 16 < 0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25 S <0.25b S <0.25 S <0.25d S <0.25d S E. coli ATCC 25922         Bacteria from purified cultures Bacteria harvested from a positive blood culture bottle Drug Phenotype CLSI MIC interpretation Macrobroth MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation ETGA MIC and interpretation gsPCR MIC and interpretation         4 hr 6 hr 22 hr 4 hr 6 hr 22 hr 4 hr 6 hr 4 hr 6 hr Ciprofloxacin Susceptible S ≤ 1 I = 2 R ≥ 4 0.008 S 0.016 S 0.016 S 0.031 S 0.016 S 0.016 S 0.031 S 0.008 S 0.008 S 0.004 S 0.008 S Tetracycline Susceptible S ≤ 4 I = 8 R ≥ 16 1 S 0.5 S 0.5 S 1 S 1 S 1 S 1 S 0.5 S 0.5 S 0.25 S 0.5 S All MIC values are in units of μg/mL.

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