The order of cue words during each recall was the same as in the foregoing learning trial. Subjects had unlimited time for recall of the target word, LY2835219 in vivo and no feedback was provided. An additional recall test took place ~ 90 min after the encoding phase. Data from one subject were discarded, owing to ceiling performance (100% correct). In the Verbal Learning and Memory Test (the German version of the Rey Auditory Verbal Learning Test) (Helmstaedter et al., 2001),

a list of 15 semantically unrelated German nouns was orally presented five times (by a pre-recorded male voice), with each word presented for 1 s. Each presentation was followed by a free recall test (L1–L5). Immediately after the fifth run, a different word list was presented [interference list (IL)], to be recalled. After recall of the IL, participants were asked to again recall the first learnt word list. Individual free recall performance was assessed by calculating the difference between the number of correctly recalled words and the number of incorrect responses (false positives – recalling a word that did not occur in the target list; perseverations – repeating an already given correct response). In the finger sequence tapping task (Walker et al., 2002), a five-digit

sequence (e.g. 4–2–3–1–4) had to be tapped with the four fingers (excluding the thumb) of the non-dominant hand as accurately and as quickly as Selleckchem Buparlisib possible. During learning, subjects performed on 12 30-s blocks with 30-s breaks in between. During retrieval, they performed on three 30-s blocks, similarly to learning. The sequence was presented continuously on a screen. No immediate feedback was given on pressing a key, but, after each block, the number of correct sequences and the total number of tapped sequences

were presented. The parameters for tSOS were similar to those in Marshall et al. (2006). The stimulating current oscillated between 0 and 250 μA at a frequency of 0.75 Hz. Anodal electrodes (10 mm in diameter) were positioned bilaterally at F3 and F4 (according to the 10–20 system), and reference electrodes were placed at both mastoids. The electrode PD-1 inhibitor resistance was < 5 kΩ. The maximum current density at the stimulation sites reached ~0.318 mA/cm2. tSOS began after 4 min of the first occurrence of continuous non-REM sleep stage 2, and consisted of six to eight 4-min stimulation epochs during non-REM sleep. The number of 4-min stimulation epochs depended on the individual subject’s sleep, as we aimed to apply tSOS only during non-REM sleep. Stimulation periods were separated by stimulation-free intervals of at least 1 min. During these stimulation-free intervals, online sleep scoring was performed to ensure that subjects still showed non-REM sleep stage 2 or SWS. If not (that is, the participant was awake or in sleep stage 1), stimulation was delayed until the subject had again entered non-REM sleep stage 2 for 2 min.