3 The current WHO classification divides B-ALL into groups based on the frequently found cytogenetic findings. These groups are: (1) acute B lymphoblastic leukemia/lymphoma with hyperdiploidy; (2) acute B lymphoblastic leukemia/lymphoma with t(9;22)(q34;q11.2); BCR-ABL1; (3) acute B lymphoblastic leukemia/lymphoma
with t(v;11q23); MLL rearranged; (4) acute B lymphoblastic leukemia/lymphoma with t(12;21)(p12;q22); TEL-AML1 (ETV6-RUNX1); (5) acute B-lymphoblastic leukemia/lymphoma Inhibitors,research,lifescience,medical with t(5;14)(q31;q32); IL3-IGH; (6) acute B-lymphoblastic leukemia/lymphoma with t(1;19)(q23;p13.3); E2A-PBX1 (TCF3-PBX1); (7) B lymphoblastic leukemia/lymphoma with hypodiploidy; and (8) acute B lymphoblastic leukemia/lymphoma Inhibitors,research,lifescience,medical not otherwise specified.2 Cytogenetic abnormalities
in ALL are classified into three risk subgroups: (1) good; (2) intermediate; and (3) poor prognosis groups. High hyperdiploidy with 51–65 chromosomes and t(12;21)(p13;q22) is classified into the good-risk subgroup and is predominantly observed in children. Also, t(9;22)(q34;q11), a representative karyotype in the poor-risk subgroup, is primarily found in adults.4 The frequency of chromosomal abnormalities varies among populations, and this difference may be due to ethnicity and geographic factors.5 It is well known that cytogenetic data Inhibitors,research,lifescience,medical are vitally important in the diagnosis, treatment, and estimation of prognosis in ALL, especially Inhibitors,research,lifescience,medical in the pediatric group.2 Nonetheless, there are only a few reports from Iran on the frequency of leukemia karyotype
abnormalities.6 In this study, we report cytogenetic findings on 168 cases of ALL patients in Fars province and compare the distribution Inhibitors,research,lifescience,medical of cytogenetic abnormalities between children and adults. The results from this study regarding the frequencies of cytogenetic abnormalities in ALL patients can be used for the classification of ALL according to the WHO groups, prognosis estimation, treatment decision, and research purposes. Materials and Methods From March 2010 to August 2012, we reviewed all cases with a final diagnosis of ALL including 154 cases of B-cell type and 14 cases of T-cell type. Definite much diagnosis in all the cases was established based on morphology, cytochemistry, this website immunohistochemistry, and flow cytometric analysis in our center. All the cases were referred from affiliated hospitals in Shiraz University of Medical Sciences. Pretreatment bone marrow aspirations or peripheral blood samples were cultured. Briefly, the samples were cultured in RPMI 1640 basal medium, containing 10% fetal calf serum (Gibco-Invitrogen-USA), for 72 hours at 37°C, and then treated with 0.1 microgram/ml of colcemid (Gibco-Invitrogen-USA) to stop the cells in the metaphase of mitosis. After harvesting with hypotonic solution (0.