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Blood 2006;107:642�?50. Ali et al. Page 10 J Immunol. Author manuscript; available in PMC 2009 February 16. UKPMC Funders Group Author Manuscript UKPMC Funders Group Author Manuscript FIGURE 1. Impact of genetic inactivation of p110γ or p110δ on mast cell numbers and vascular permeability responses in vivo. A, Mast numbers in different anatomical locations. Depending on the anatomical location, the units for counting were as follows: ear dermis: per 5 mm length, beginning at the ear tip; stomach: per one complete sagittal section; and back dermis: unit is per 10 high power fields. Exclusively, dermal mast cells were counted for each mouse; s.c. mast cells were not counted, as thickness of the s.c.
fat can vary greatly among animals depending on body condition. Data are presented as mast cell numbers expressed as % of WT. The mast cell distribution in δD910A mice has been published previously and is presented here for comparative purposes. B, Effect of vasoactive stimuli on vascular leakage in PI3K mutant mice. Numbers of mice used were as follows: histamine: WT, γKO, and δD910A, n _ 6 each; and mast cell extract: WT, n _ 8; γKO, n _ 6; and δD910A, n _ 8. Ali et al. Page 11 J Immunol. Autho