Selected hlt On the basis of work in DNA-PK Inhibitors which neurochemical and behavior Changes were recorded. 4.5. Behavioral protocol 4.5.1. Auto Shaping auto shaping task learning framework or sign-tracking, a hungry animal is placed in a climatic chamber in order to find food pellets in the food shop and then a sequential Pavlovian pair of a key or a retractable lever, where illuminated light and food. After a series of these Pr Presentations, n To hert the animal represents the CS and instrumental responses, such as Peck, nose bag, and the shift lever of the press. Then CR or car shaped response results of the association and SS are supported by the club capture. It is in pursuit of the development progress Verhaltensst Important task requirements, a place P / IA combines both Pavlovian and instrumental conditioning. These offer the M Opportunity to study the hippocampus mediate declarative Ged MEMORY and habit formation striatummediated RS. In addition, P / IA, with the exception of the education magazine nearly YOUR BIDDING automated, thus significantly reducing human intervention. It is sensitive to small erh Relationships or decreases in the different behavioral parameters, including normal signs monitoring, and monitoring goals. The latter is very important because they can study the bi-directional expression of a training or an extended memory adversely Are made more prominent k. P / IA clearly separates training for testing sessions, and it was useful for the detection of supply Changes in the Ged Chtnisbildung induced by drugs or aging. 4.5.2. Food magazine and auto shaping individual training was each rat in an experimental chamber for a Eingew Placed hnungszeit ft with access to 50 food pellets before in the Lebensmittelgesch. The criterion was that once the animal has eaten all 50 food pellets and pr Presents 150 w nose Highest in the supermarket, the training program was initiated in auto shaping. The program had been reported before auto shaping, and it consisted of discrete trials.
A trial began with the Press Presentation of a retractable lever and illuminated for 8 s followed by a supply of food pellet. There was a testing time interval between 60 s when the animal pressed the CS, there was a conditioned reflex, which shortens the process, the lever back, turned off the light, and considered delivered aUSwas. The Erh Increase or decrease in CR was considered as an index to improve or adversely Chtigungen in memory consolidation, respectively. It was a training session lasted about 12min auto shaping and three training / test sessions, each about 24min. All sessions were conducted on three consecutive days. The training was followed by consecutive car MEMORY shaping training / testing sessions at 1.5 h for the Kurzzeitged Memory, and 24 and 48 h for the Langzeitged. In addition st T increases or decreases in head / CS as a measure were used for the motivation for the food pellets and exploratory behavior. 4.6. Determination of GSK3 and total GSK3 phospho 4.6.1. Tissue preparation after the last training auto shaping / CB test session, and STZ groups STZdrugs, and CB untrained rats by decapitation get Tet and for p determined GSK3 and total GSK3 levels. The brains were rapidly removed, placed on ice and the pr Frontal cortex and hippocampus for each group dissected after Paxinos and Watson. Coordinates for the pr Frontal cortex of the bregma.