Effects on efficacy, tolerability, and satisfaction were reported as mean between-group differences with 95% CIs. The number of participants reporting adverse events was calculated as percentages for each arm of the study. The number of participants who preferred each timing regimen was reported as a proportion. Adherence was calculated

as the total number of airway clearance sessions performed divided by the total number of sessions scheduled, LBH589 solubility dmso and reported as a percentage. Fifty of the 52 patients approached about participation in the study gave consent and were eligible for the study. All 50 participants completed the three days of interventions as randomised. After completion of this initial data collection, each participant was followed for one year, during which

14 participants were re-admitted to hospital for a respiratory exacerbation. All 14 participants again met the eligibility criteria and agreed to repeat the three-day study. All 14 participants completed the three days PFI-2 of interventions as randomised. The flow of participants through the trial is illustrated in Figure 1. The characteristics of the 50 initial participants are presented in the first column of Table 1. The comparability of the participants’ clinical condition at baseline on each of the three study days is shown in the first three columns of Table 2. Additionally, the average study day on which each regimen was experienced was study day 2 (SD 1) for all three regimens, indicating successfully balanced allocation of treatment orders. The range of techniques used included modified postural drainage and percussion (n = 35), positive expiratory pressure (31), oscillating positive expiratory pressure (4), autogenic drainage (5), and active cycle of breathing techniques (28) (Pryor and Prasad 2008). The Thiamine-diphosphate kinase total is greater than 50 because some participants used a variety of techniques

in their airway clearance session. The range of techniques for each individual participant remained standardised over the three study days. The characteristics of the 14 participants who repeated the study are presented in the second column of Table 1. Their characteristics were typical of the initial cohort of 50 participants except their lung function was lower, whichis consistent with their readmission to hospital. The mean time between both studies was 295 days. The content of the treatment session, including tailoring of the airway clearance techniques and confirming the appropriate nebulisation procedures, was determined by the Cystic Fibrosis Unit physiotherapist, who had 20 years of clinical experience, including 17 years in the cystic fibrosis area. The Cystic Fibrosis Unit of Royal Prince Alfred Hospital, which manages approximately 250 adult patients, was the only centre to recruit and test patients in the trial.