However, the causative roles of Gd remain unknown.
Objective: The aim of this in vitro study was to investigate the effect of Gd on the development of fibrosis and calcification in cultured cells.
Methods: MC3T3-E1 cells (pre-osteoblastic cells), human adipose tissue-derived mesenchymal stem cells (HAMSCs), human subcutaneous preadipocytes,
and human dermal fibroblasts (HDFs) were PLX3397 datasheet each cultured in differentiation medium with or without gadolinium chloride. Calcium deposition of MC3T3-E1 cells, HAMSCs, and HDFs was determined by alzarin red S staining. Adipogenic differentiation of human subcutaneous preadipocytes and HAMSCs was determined by oil red O staining. Fibrogenesis of HDFs was determined by real-time PCR to measure the mRNA expression of type I collagen. Cell proliferation was determined by MTS assay.
Results: Gd induced calcium deposition in MC3T3-E1 cells, HAMSCs and HDFs in osteogenic differentiation media. Gd did not induce adipogenic Selleck Belnacasan differentiation in human subcutaneous preadipocytes and HAMSCs. Gd did not increase the mRNA expression of type I collagen in HDFs, but did promote cell proliferation.
Conclusions: We have demonstrated a direct effect of Gd on calcium deposition in cultured cells. The result will help us to understand the mechanism of abnormal calcification in
NSF. (C) 2011 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.”
“A rare complication of mature cystic teratoma of the ovary is malignant transformation, mostly being squamous cell carcinoma (SCC). Due to small case series, there are no established standard surgical and postoperative procedures.
A 41-year-old pregnant woman presented with a right ovarian tumour. At 14 weeks of gestation, laparotomy with oophorectomy was performed. Histology revealed a pT1a poorly differentiated SCC
with lymphovascular invasion. She delivered a healthy infant at 39 weeks of gestation. Nine weeks after delivery, Selleckchem DAPT she underwent complete surgical resection and was staged pT1a, pN0 (0/40) pMx, G0. No further treatment was given and 37 months later she is still free of disease.
For this rare entity complete surgical resection seems to be a safe treatment.”
“Background: Polycystic ovary syndrome (PCOS) is the most common chronic endocrine disorder affecting women of reproductive age. This study aimed to compare the HRQoL of South Asian and white Caucasian women with PCOS, given that it is particularly common among women of South Asian origin and they have been shown to have more severe symptoms.
Methods: The Polycystic Ovary Syndrome Questionnaire (PCOSQ) and the Short Form-36 (SF-36) were administered in a cross-sectional survey to 42 South Asian and 129 Caucasian women diagnosed with PCOS recruited from the gynaecology outpatient clinics of two university teaching hospitals in Sheffield and Leeds.