In addition, the Haber-Weiss reaction, an interaction between OHN O. form {2 and H2O2 in the presence of Fe2 + or Fe3 +. These oxygen radicals and H2O2 are discussed as reactive oxygen species. DNA Lenvatinib strand breaks caused by free radicals in these reactions. Depending on their concentration, H2O2 PLoS ONE | Published in PloSOne first April 2009 | Volume 4 | Issue 4 | e5131 induces two types of DNA-the Sch: DNA single-and doppelstr Independent. Single-stranded DNA are within the meaning of H2O2-stress dominant, but this L Emissions are repaired efficiently and does not appear to mediate the cytotoxic reaction. On the other hand, DNA double-strand breaks in H2O2 stress rarely occur, but they are potentially toxic and induce apoptosis. Moreover, after IR stress, are DNA double strand produced and the proteins ATM phosphorylated.
The actual mechanism of this process is not yet understood. However, one of the targets of ATM phosphorylation suggested that the Nbs1 protein, which is to be with the conserved DSB repair factors Mre11 and Rad50. The phosphorylated ATM phosphorylates itself, and it is proposed that XL880 ATM breaks within 15 minutes after exposure to 0.5 Gy IR autophosphorylated comprising the DNA induces 18 approx, Hr However, it was not known, ATM detects a small number of Bezirksschulr-run and activates signaling cascades. In this paper we propose a mathematical model of the process of ATM phosphorylation on a stochastic generation of a small number of Bezirksschulr-run independent Ngig of the source of the damage.
In our model we assume that CBD binding by DNA-Sch To be generated, the repair proteins in order to become and DSB-RP complexes, and the CBD are repaired. CSD and DSBCs are very small, and these processes are stochastic. We will see that we are theoretical values for the mean number of CSD and DSBCs on the basis of the number of repair proteins And rate constants of the repair charge. The Bezirksschulr-run products and DSBCs phosphorylate ATM which autophosphorylates. We will see that CBD repair is not successful and the number of DSB increases with the number of repair proteins Is small, but if enough repair proteins, the number of DSB is suppressed at low levels. In addition, we found that autophosphorylation of ATM induces bifurcation of ATM kinase.
Monostable, bistable reversible and irreversible bistable diagrams: according to the total concentration of ATM, the fixed points of ATM * Three types of diagrams of stable states his ends. These diagrams station Ren Bistabilit t appears t, where the total concentration of ATM increases, and the concentration of the ATM switch has * – Similar behavior in the presence of these bistabilities. In addition, we see that the detection time after DNA-Sch Ending as the total concentration of ATM decreases. Results Figure 1 shows a schematic of our model. CSD can be induced by some stress signals, and the repair proteins Bind to CSD, producing DSBCs. The plant produces a repaired DSB repair. We model these processes as stochastic processes. The details in the n Next section. CSDs and strengths generated by DSBCs ATM, and is phosphorylated to the verst stress.
In this paper we focus Haupts Chlich on the DSB repair processes and the mechanism for detection of ATM. Negative feedback of p53 is also addressed in the discussion. Calculation methods for DSB production model in this paper, we assume that the following terms of a stochastic mechanism of production of DSB and the repair process: 1 DCA C1 DSBzRP DSB / C2Z C2-C3 DsbC DsbC DCA RDSBzRP E1T where does DNA DSB breaks doppelstr Independent, called the RP-repair proteins And DsbC represents DSB repair protein complexes and RDSB means the DSB repair. The constants c1, CZ 2,