Each compound was Nelarabine DNA Synthesis inhibitor embedded a gel-free tissue treated accordingly. All samples contained the same amount of ANF, PBS, and MeOH. The samples were stored at 40 ° C until analysis. 2.9. The desorption of drugs from the polyacrylamide gel was washed, the tissue fixed at 10.0 mL of human plasma or whole blood of 37 ° C, transferred and stirred gently with a rocking platform shaker for 1 h at 37 C. 1.0 ml samples were within 15 , drawn 30 and 60 min. The sampled volume was replaced with fresh human plasma are vorgew RMTE or whole blood. The samples were stored at 40 ° C until analysis. 2.10. Sample preparation of the sample analysis and HPLC conditions adsorption experiments were for 30 min at 13,000 g at 10 ° C. The centrifuged whichever type Walls were injected directly into the system of high performance liquid chromatography. Typically, 50 lL sample was injected. The linearity was t given 10-500 ng / mL for FP, Bud, and AZ, and the correlation coefficients of calibration curves were at least 0.99 s. Plasma samples of 1.0 ml with a L Solution of 50 lL internal standard mixed and washed twice with 3 ml of diethyl ether for 20 min with a roller mixer by centrifugation at 800 g for 1 min at room temperature. The organic layers were combined and evaporated to dryness under a gentle stream of nitrogen at 25 ° C. The resulting residue was reconstituted in methanol. AZ of blood samples of 1.0 ml with 50 lL internal byaddition Standardl Solution of 250 lL NaOH mixed followed. Min after two extractions with 4.5 ml of hexane / n-octanol for 20, the samples were at 2000 g for 5 min centrifuged at room temperature. The organic phases were combined, incubated with 150 lL of 0.2% acetic Acid, and vortexed for 2 min. The w Aqueous phase was used for injection into the HPLC system directly. Typically 20 lL sample was injected. The linearity was t given 10-200 ng / ml for FP and 10 400 ng / mL for Bud and AZ, and the correlation coefficients of calibration curves were at least 0.99 s. The HPLC system is an HPLC pump water in 1525 consists of an I Ren, an autosampler 717 plus in 2487 and two wavelength detector Absorption lengths. The analysis was performed on a Symmetry C18-S Molecules for glucocorticoids And the Lichrospher 100 CN. Data collection and integration were 鈩 Breeze Version 3.4 software. A rate of 1 ml / min, and Detektionswellenl Length was set at 254 nm for FP and Bud. AZ was at a flow rate of 0.75 ml / min, wavelength Analyzed set length and detection at 210 nm. The mobile phase for glucocorticoid Consisting of water containing 0.2% acetic Acid and acetonitrile. For FO, the gradient began 60:40 A / B increased linearly to 29:71 min A / B over 30. For Bud, the gradient began 60:40 A / B increased linearly to 40:60 min A / B over 20. The mobile phase for elution was isocratic AZ a composition 50:50 acetonitrile and water as described above. 2.11. The inhibition of IL-8 secretion by plasma samples after desorption of tissue Lopinavir Proteasome inhibitor samples from polyacrylamide gel of desorption of the gel were obtained also used for incubations cell culture. In the case of experiments, AZ, were new adsorption / desorption performed and the plasma was used instead of whole blood. For all drugs studied, the plasma was supple.