Of interest is the potential real world application of this study considering all of the participants Go6983 mw were habitual caffeine consumers with a moderate
daily intake of caffeine (<200 mg/day) and were still responsive to the active supplement treatment. This regular intake of moderate amounts of caffeine may explain much of the lack of observed hemodynamic and ECG effects in this investigation. Tolerance to caffeine can develop within four days of consuming 150 mg/day  and this built-up tolerance can negate or reduce the side effects often seen when a non-caffeine user ingests a caffeine-containing beverage/supplement including increases in SBP, DBP, and changes in HR . In addition to a lack of negative physiological side effects, participants also did not report any negative mood states or other side-effects. When participants were given 280 mg of caffeine in the form of coffee, Smits and associates  observed an increase in BP and a decrease in HR, while there were no significant changes among the control group (decaffeinated coffee). These changes in HR ABT-737 cell line and BP were assumed to be linked to the caffeine content of the regular coffee. Considering the supplement used in the present study contained 340 mg of total caffeine, habitual moderate caffeine usage seems to be the contributing factor to no significant changes in HR, BP, and ECG
data, as well as the lack of reported side-effects. Conclusion In conclusion, when taken by moderate caffeine users that are physically active and healthy, the proprietary blend of this particular thermogenic supplement can increase REE and mood states related to alertness, focus, and energy without causing unsafe acute hemodynamic side-effects or increasing selleck chemicals perceived anxiety levels. Future research Arachidonate 15-lipoxygenase should evaluate the chronic combined effects of DBX with exercise. Acknowledgements We would like to thank all of our participants for volunteering
for the study as well as all of the research assistants in the HPL that assisted with data collection. We would also like to thank Dymatize Nutrition for sponsoring this study. References 1. Dalbo VJ, Roberts MD, Stout JR, Kerksick CM: Acute effects of ingesting a commercial thermogenic drink on changes in energy expenditure and markers of lipolysis. Journal of the International Society of Sports Nutrition 2008, 5:6.PubMedCrossRef 2. Kreider RB, Wilborn CD, Taylor L, Campbell B, Almada AL, Collins R, Cooke M, Earnest CP, Greenwood M, Kalman DS, Kerksick CM, Kleiner SM, Leutholtz B, Lopez H, Lowery LM, Mendel R, Smith A, Spano M, Wildman R, Willoughby DS, Ziegenfuss TN, Antonio J: ISSN exercise & sport nutrition review: research & recommendations. Journal of the International Society of Sports Nutrition 2010, 7:7.PubMedCrossRef 3. Roberts MD, Dalbo VJ, Hassell SE, Stout JR, Kerksick CM: Efficacy and safety of a popular thermogenic drink after 28 days of ingestion.