The embryos of fish traps and also share in an effective and uniform miniaturized drug delivery through the network. The F Ability, tests of continuous infusion, without result, the embryos PDK1 is an important consideration in the Kotoxikologie in particular. The static tests conducted usually bo Petri dishes are the easiest and cheapest way to get the toxicity of t to assess chemicals. However, they are insufficient to eliminate the toxicity of t many compounds because of their adsorption, degradation, metabolic inactivation, oxygen deficiency, uncontrollable Ver Changes POSE to test pH of the medium. All these factors hamper k Can seriously the relevance of the static display standard tests used in FET. Therefore, it has been postulated recently by Lammer et al, the cross or the acute toxicity tests dynamic t to be the preferred choice for the analysis of toxic substances.
Furthermore, as Lammer et al Yet another disadvantage is the static Fludarabine FET that these quantities not to use very small amounts of test substance required to Best Account the results chemicals U Only difficult. Be removed by the formation of a perfusion system k Can gr Ere volume of the medium for chemical analysis Best Confirmation test such as the metabolic degradation of compounds. This should be green Erer contr The quality, and the data for a comprehensive interpretation of the toxicity of t. In addition, the device t M Possibility that embryos with the drug through the medium of exchange and rapid pulse followed embryo without the position.
This may be of particular importance for the controlled L is precisely the transcriptional activation of target genes using inducible transgenes, such as tetracycline inducible Tet on Gene Expression System. In addition, k The drug delivery pulse can for the delivery of toxins temporary and / or An Sthetika to short-term exposures to high doses that are used to assess the development of the embryo in a medium without drugs followed. The delivery of cells durchl, precious metals, fluorescent probes can be considerably fast by washing their performance to the background fluorescence in the subsequent reduction Takeover easier. Long-term micro-environment of the embryo culture to demonstrate the feasibility of analyzing the development of Zebrab Rbling for long ZEITR dreams, be carried out numerical simulations in addition to both the beaches flow velocity abzusch COLUMNS and magnitude the shearing forces is applied to the embryos.
Rst Was a 3D model of the entire system built and the analysis of the flow profile of Computational Fluid Dynamics simulations on the device T get at full load. The embryos were simulated as a rigid, non-deformable Sph Ren. The distribution of shear stresses in the traps was then obtained from a 3D model. The results showed that embryos experienced an average shear stress of 2 to 1.83E 4.60E 2 Pa at the infusion of 0.4 ml / min. A maximum shear stress of 2 Pa 6.13E should be known disposed through the embryos in the first case each row. This suggests that held by the nature of the river within the miniaturized system for capturing the embryos in a low shear stress microenvironment. In this regard, we have studied in detail the signaling associated with individual cells in a range of flow-induced mechanical stresses. Our current results indicat