Published by the Animal Care and Use Committee Institutional Research at the Institute of Nuclear Energy, Taiwan. Preparation of 188 Re labeled liposomes BMEDA pegylated pegylated liposomes have been described using the method of Tseng et al.21 The lipid compositions of liposomes containing soy phosphatidyl choline hydrogen, SGX-523 1022150-57-7 cholesterol, polyethylene glycol and an L Solution of ammonium sulphate with water in the inner phase. Nanoliposomes pegylated an average particle E nm of about 82.59 13.16 and on ole / ml phospholipids. A method for radiolabeling with 188Re was BMEDA as already briefly described.22, were BMEDA was tin chloride is used as reducing agent and glucoheptonate used as an intermediate ligand to 188Re SNS / S complex. Five milligrams BMEDA were in a new vessel pipetted.
A volume of 0.5 ml 0.17 mol / L glucohepatonate in an L Solution MLN8237 1028486-01-2 of 10% acetate gel St added followed by addition of 120 tin chloride. Rin by lacing the L Solution with N 2 gas, a high specific activity Added tons of sodium perrhenate 188Re. The vessel was closed and in a water bath 80 for 1 hour. The efficiency of the labeling BMEDA 188Re complex was purified by silica gel glass fiber sheet packs with normal saline Analyzed as a developer solution. The liposomes were pegylated nano X 188Re to the L Solution and incubated at 60 BMEDA for 30 minutes. The liposomes were separated from 188Re 188Re free BMEDA over a PD 10 with physiological saline Eluted solution. Each 0.5 ml fraction was in an R Hrchen collected. The opacity of pegylated liposomes was used contr L visual collection liposomes 188Re.
The labeling efficiency was determined using the activity of t in pegylated liposomes after separation divided by the total number activity t before separation. Biodistribution and pharmacokinetic studies F��nfunddrei Of pure C26 C Lon metastatic mouse peritoneal carcinomabearing re U is an intravenous Se injection of 3.7 L of 0.88 MBq/200 188Reliposomes oil with phospholipids for biodistribution and pharmacokinetic studies. The Mice were maintained over 12 hours light / dark cycle and had free access to food and water. For biodistribution studies, 30 Mice with CO2 asphyxiation, five M Mice sacrificed at 1, 4, 16, 24, 48 and 72 hours after administration. at any point in time, the organs were sampled and collected all the organs of interest, where m is possible.
The samples were dissolved in saline Purged solution, dried and weighed, then counted using a Packard Cobra II auto gamma disadvantages. Examples of the injection of 188Re liposomes were used as decay correction standards. Data were expressed as percent of injected dose per gram tissue. For pharmacokinetics, blood samples 20-100 were collected at 0.083, 0.5, 1, 4, 16, 24, 48, 72, 120 and 168 hours of five M Mice by cardiac puncture after the intravenous injection Se liposomes 188Re . Radioactivity t concentrations in blood were as% ID / ml. The pharmacokinetic parameters were determined using the WinNonlin software. Non-compartmental analysis model 200 was used with log / linear trapezoidal rule. Including parameters Lich terminal half-life, Tmax, Cmax, total clearance and Fl Surface determined under the curve.
The pharmacokinetic parameters were associated with the terminal phase COLUMNS based on the best-fit method, the terminal half-life to beautiful. Micro SPECT / CT imaging, and all K Body autoradiography for micro SPECT / CT imaging were three Mice again U is an intravenous Se injection of 12.95 MBq/200 of 188Re phospholipid liposomes and 0.88 ol. The M were Mice bet exerts With 1.5% isoflurine and rec