The results suggested that an important role of H. parasuis OmpP2, at least in the SC096 strain, appeared to be its ability to protect against the bactericidal AZD4547 clinical trial activity of complement. Future in vivo studies are required to investigate this further. In conclusion, in this study, a modified natural transformation method in H. parasuis was developed that could provide an avenue to identify the function of different genes. Using this genetic manipulation system, the ΔompP2 mutant of the H. parasuis SC096 strain was determined to be significantly more
sensitive to serum killing than its wild-type strain. The results indicated that OmpP2 is required for serum resistance in H. parasuis SC096, belonging to serovar 4. This work was supported by the Program for New Century Excellent Talents in University (Grant No. NCET-06-0752), the Program for Changjiang Scholars and Innovative Research Teams in Chinese Universities (Grant No. IRT0723) and the Innovative learn more Research Teams Program of Guangdong Natural Science Foundation (Grant No. 5200638). B.Z. and S.F. contributed equally to this paper. “
“Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand Streptococcus suis, an emerging zoonotic pathogen, is responsible
for various diseases in swine and humans. Most S. suis strains from clinical cases possess a group of capsular polysaccharide synthesis (cps) genes and phenotypically express capsular polysaccharides (CPs). Although CPs are considered to be an important virulence factor, our previous study showed that many S. suis isolates from porcine endocarditis lost their CPs, and some of these unencapsulated isolates had large insertions or deletions in the cps gene clusters. We further investigated 25 endocarditis isolates with no obvious genetic alterations to elucidate the unencapsulation
Edoxaban mechanisms and found that a single-nucleotide substitution and frameshift mutation in two glycosyltransferase genes (cps2E and cps2F) were the main causes of the capsule loss. Moreover, mutations in the genes involved in side-chain formation (cps2J and cps2N), polymerase (cps2I), and flippase (cps2O) appeared to be lethal; however, these lethal effects were relieved by mutations in the cps2EF region. As unencapsulation and even the death of individual cells have recently been suggested to be beneficial to the pathogenesis of infections, the results of the present study provide a further insight into understanding the biological significance of cps mutations during the course of S. suis infections. “
“Klebsiella pneumoniae carbapenemase (KPC)-encoding genes containing promoter-deletions (blaKPC-2a, blaKPC-2b, and blaKPC-2c) have disseminated in Enterobacteriaceae. The minimal inhibitory concentrations (MICs) to β-lactams in clinical KPC-producing Enterobacteriaceae range from susceptible to high-level resistant, resulting in diagnostic problems.