They observed impor tant differences meantime in the levels of key regulators of the cell cycle, signal transduction, apoptosis, transcriptional regu lation, Inhibitors,Modulators,Libraries and cell metabolism. The benefits of using cancer cell lines in cancer research are clearly demonstrated by the In Vitro Cell Line Screen ing Project at the National Cancer Institutes Developmental Therapeutics Program The IVCLSP screens up to 3,000 drug compounds every year for poten tial anticancer activity, using 60 different human tumor cell lines, representing leukemia, melanoma and cancers of the lung, colon, brain, ovary, breast, prostate, and kid ney. The success of this program can only mean that cell lines do provide valuable biological data, and sometimes, accurate representation of the in vivo biology of tumors.

The present study was designed to employ multidimen sional protein identification technology pro teomics, Gene Ontology mapping and directed acyclic graph representations to detect, compare and contrast qualitative and quantitative oxidoreductase enzyme expression profiles of the HepG2 proteome vs. that Inhibitors,Modulators,Libraries of a normal human liver. Two independent sets of bioinformatics calculations that employ two BLAST program versions, and searched differ ent sets of databases, arrived at essentially the same con clusion that oxidoreductases are down regulated in HepG2 cells by an average of 57%, when compared to normal human liver tissues. Methods Samples HepG2 cell lysates, 500g in 0. 5 mL in SDS PAGE Buffer, and normal human liver tissue lysates, 150g in 0.

032 mL denaturing buffer with Inhibitors,Modulators,Libraries pro teolytic inhibitors to minimize proteolytic damage to pro teins, all lysates extracted by the method of Laemmli, were obtained from RDI Division of Fitzgerald Industries Intl and stored at 80 C until use. Prior to use, the lysates are aliquotted into 100g total protein portions and stored at 80 C. A 100g aliquot is used for each experiment. Under the auspices of Virginia Commonwealth University Office of Research Subjects Inhibitors,Modulators,Libraries Protection, compliance with U. S. Department of Health and Human Services regulations at 45 CFR 46. 101 was provided by Fitzgerald Industries. 2D cleanup Proteins are separated from buffers, Inhibitors,Modulators,Libraries detergents, salts and other contaminants using a 2D clean up kit and protocol provided by Amersham Biosciences. The kit consists of four reagents a Crizotinib c-Met inhibitor precipi tant that pellets the proteins, a co precipitant that enhances the removal of the proteins from the solution, a wash buffer that removes non protein contaminants from the protein precipitate, and a wash additive that promotes rapid and complete re suspension of the proteins. Prior to the beginning of clean up, the wash buffer was chilled at 20 C for 1 hr.