Distribution of HLA ligands C1 (HLA-C, 80N), C2 (80K), Bw4 (HLA-B, 80I or T and HLA-A*2301, 2402 and 3201) and HLA-A3/A11 did not differ significantly between DILI patients and controls. The most frequent receptor-ligand combinations in the DILI patients were 2DL3 + C1 (67%) and 3DL1 + Bw4 (67%), while 2DL1 + C2 (69%) and 3DL1 + Bw4 (69%) predominated in the controls. In contrast, 3DS1+Bw4 was the least frequent receptor-ligand combination in DILI (9%) and controls (11%). Conclusion: This is to our knowledge the first KIR association analysis in DILI patients. However, our AC DILI cohort presented KIR gene distributions similar to the controls, which were comparable to previously

reported KIR data from RGFP966 CDK inhibitor drugs ethnically similar cohorts. Furthermore, the analysed

KIR receptor–HLA ligand combinations do not appear to play a major role in AC DILI development. The complete ligand repertoire is however not elucidated and a potential role for KIR in AC DILI should not yet be dismissed. Funding: PI-0239-2012 SAS, FIS PI12-00378, AC-0073-2013 SAS, CIBERehd by ISCIII Disclosures: Manuel Romero-Gomez – Advisory Committees or Review Panels: Roche Farma,SA., MSD, S.A., Janssen, S.A., Abbott, S.A.; Grant/Research Support: Ferrer, S.A. The following people have nothing to disclose: C. Stephens, Antonia More-no-Casares, MAngel López-Nevot, Miren García-Cortés, I. Medina-Cáliz, Hacibe Hallal, German Soriano, Francisco. Ruiz-Cabello, M. I. Lucena, Raul J. Andrade [Background and Aim] The recent global increase in the incidence of metabolic syndrome has also impacted its hepatic manifestation

in the form of an increased prevalence of non-alcoholic fatty liver disease (NAFLD). It is known that liver metabolism is regulated by a ‘metabolic highway’ mediated by the autonomic nervous system. The component neurons are distributed within the liver, extending from the portal area, and then gradually branching to form a fine network around the portal area. However, the precise mechanism by which this regulatory system operates is still poorly understood. Therefore, the aim of the present study was to examine the role of the autonomic nervous system in the liver using immunohistochemistry, and to clarify the association between these nerves and a variety of liver diseases. [Methods] First, we evaluated Adenylyl cyclase the autonomic nervous system in the liver after transplantation, which in theory should lead to intrahepatic neuron atrophy. As neuron markers, we evaluated changes in S-100 or N-CAM immunostaining over time (n=90). Specimens of normal liver obtained at surgery for metastatic liver cancer were used as immunostaining controls (n=5). Also, we evaluated a diverse group of liver diseases (NASH n=18, chronic hepatitis B n=10, chronic hepatitis C n=10) to evaluate whether these diseases show differences in the ratio of positivity for neuron markers.