Liver biopsies were read and scored according to NASH CRN scoring system. The associations of premature menopause and the time from menopause with fibrosis severity (stage 0-4) were assessed using ordinal logistic regression models with and without adjusting for age at enrollment, race/ethnicity, waist circumference, current smoking/alcohol use, hypertension, diabetes, Rapamycin mw HOMA-IR, and hormone replacement therapy (HRT). [Results]
Mean age ± SD at menopause was 44 ± 9 yrs. 29.5% women had premature menopause. This group was younger at enrollment (55 ± 9 vs. 59 ± 7 yrs, p<0.001, t-test) and used HRT more often (32% vs. 19%, p<0.003, Chi-square test). Premature menopause was associated with a higher stage of fibrosis (p<0.05, Wil-coxon rank-sum test).
No significant differences were noted in other histologic features or above-listed clinical variables. After adjusting for age at enrollment, premature menopause was associated with an increased likelihood of having more severe fibrosis; cumulative odds ratio and 95% confidence interval (COR[95%CI]) was 1.7[1.2, 2.4] (p< 0.004), which remained similar after adjusting for the other variables. Time from menopause was also directly associated with an increased likelihood of having more severe fibrosis (COR[95%CI] for 5-year unit=1.2[1.1, 1.3], p<0.0001), which also remained the same after adjusting for the other variables. [Conclusion] Age at menopause was significantly associated with a risk of fibro-sis among post-menopausal BGJ398 women with NAFLD. This finding, together with our previous reports, underscores the significance of reproductive ADAM7 information for risk stratification among female patients with NAFLD. Disclosures: Manal F. Abdelmalek – Consulting: Islet Sciences; Grant/Research Support: Mochida Pharmaceuticals,
Gilead Sciences, NIH/NIDDK, Synageva, Genfit Pharmaceuticals Joel E. Lavine – Consulting: Merck, Crosscare, Gilead, Takeda Millenium; Grant/ Research Support: Janssen Anna Mae Diehl – Consulting: Roche; Grant/Research Support: Gilead, Genfit The following people have nothing to disclose: Jagpal S. Klair, Ju Dong Yang, Cynthia D. Guy, Ryan M. Gill, Katherine P. Yates, Aynur Unalp-Arida, Jeanne M. Clark, Ayako Suzuki Background: Experimental evidence suggests a cardiopro-tective role of heat shock proteins (HSP) in several models of acute myocardial stress. Patients with both non-alcoholic fatty liver disease (NAFLD) and coronary artery disease (CAD) have lower levels of serum HSP. HSP may stimulate autoimmune responses resulting in the production of antibodies. Aim The aim of this study is to investigate the possible role of anti-HSP auto-antibodies in reduction of HSP in patients with NAFLD, leading to increased prevalence of coronary artery disease (CAD). Methods: We prospectively enrolled 119 patients undergoing elective coronary angiography. Each patient had fasting serum, clinical data and abdominal ultrasound (US). All US were read centrally by a standard protocol.