Only proteins with q-value below 0.05 or those present in only one of two compared analytical groups were taken into consideration during further analysis. The protein concentration was measured by Bradford’s method [35]. Results and discussion Batch fermentation Microbiological synthesis of 1,3-PD by C. butyricum DSP1 was carried out at an increasing capacity of bioreactors. The efficiency of 1,3-PD production from crude glycerol during the scale-up process

was investigated. For this purpose batch fermentations were performed in 6.6 L, 42 L and 150 L bioreactors. The results obtained were used to calculate the basic kinetic parameters of the fermentation processes (Table 2). It was found that the scale-up process did not have any effect BMN 673 ic50 on the growth of microorganisms or 1,3-PD synthesis. Table 2 Kinetic parameter values from C. butyricum DSP1 in 6.6 L, 42 L and 150 L bioreactors Parameter/fermentation scale 6.6 L 42 L 150 L Time of fermentation (h) 33 32 28 Max biomass, Xmax (g/L) 1.2 1.2 1.3 Time taken to reach max biomass, t (h) 15 16 14 Max specific growth rate, μ (1/h) 0.067 0.062 0.071 Max 1,3-PD concentration, selleck screening library 1,3-PDmax (g/L) 37.63 ± 1.2 36.40 ± 1.6 37.20 ± 1.4 1,3-PD productivity P1,3-PD (g/L/h) 1.12 1.13 1.33 1,3-PD yield, Y1,3-PD (g1,3- PD/gGly) 0.53 0.52 0.53 Max butyric acid

concentration, Butmax (g/L) 4.26 ± 0.09 3.57 ± 0.08 GPX6 4.22 ± 0.07 Butyric acid productivity PBut (g/L/h) 0.13 0.11 0.15 Butyric acid yield, YBut (gBut/gGly) 0.06 0.05 0.06 Max acetic acid concentration, Acemax (g/L) 2.0 ± 0.03 1.9 ± 0.03 2.2 ± 0.02 Acetic acid productivity PAce (g/L/h) 0.06 0.06 0.08

Acetic acid yield, YAce (gLac/gGly) 0.03 0.03 0.03 Max buy Lazertinib Lactic acid concentration, Lacmax (g/L) 3.14 ± 0.02 2.84 ± 0.03 3.63 ± 0.04 Lactic acid productivity PLac (g/L/h) 0.09 0.09 0.12 Lactic acid yield, YLac (gLac/gGly) 0.04 0.04 0.05 Each point is the mean value of two independent measurements. The concentration of the diol in the 150 L bioreactor was close to concentrations achieved in the 6.6 L and 42 L bioreactors and averaged 37 g/L. In all batch fermentations the glycerol was completely utilized. However, some differences were observed in the productivity of the bioreactors as their capacity increased, with the 150 L bioreactor giving 1.33 g/L/h, which probably depended on the quantity of biomass (Table 2). The plateau of microorganism growth was achieved in the14th hour of cultivation and was followed by the stationary phase. The profiles of by-products formed in the respective bioreactors were comparable. The first scale-up experiments on 1,3-PD synthesis from glycerol (by C. butyricum DSM 5431) were described by Günzel et al. [24] and involved fermentation starting in a 1.4 L bioreactor and proceeding to a 2000 L bioreactor.