There are no studies of comparative genomics in Rhizobiales with

There are no studies of comparative genomics in Rhizobiales with a focus on symbiosis and pathogenesis processes with the analyzed BTSA1 mouse representative mTOR inhibitor species of both lifestyles and showing phylogenetic analysis with many distinct operons involved in these processes. Besides this, the database offered by this study is the most representative for Rhizobiales until now and will also allow further important

investigations that may help to infer crucial events that had contributed to the evolution of symbiosis of pathogenesis interactions. Methods In order to select the species used for genomic comparison based on their phylogenetic proximity, a reconstruction with 30 bacteria belonging to the order Rhizobiales was obtained. The chosen this website strains belong

to 25 different species and 12 genera and are shown in Figure 1. The reconstruction was performed by using a dataset consisting of 104 concatenated housekeeping proteins [55] based on the work of Williams et al. (2007) [56] and kindly provided by the authors, which showed a robust reconstruction for alpha-Proteobacteria. In addition to the species used by these authors, we included the sequences of R. vitis strain S4 and R. radiobacter strain K84, both previously classified in the genus Agrobacterium and both of whose genomes are available: strain S 4 is the pathogenic agent of crown gall disease in grapes, while strain K84 is non-pathogenic and has been developed for worldwide commercial use to control crown gall. The tree generated was then established as the model phylogeny. From this tree, species with the largest phylogenetic proximity with the neighbor species of the other genera were selected, and representatives of the beta-Proteobacteria class were used as the outgroup. Therefore, from the 30 species used in the reconstruction model (Figure 1), 19 were selected for comparative analysis (additional file 1). Rhizobium sp. NGR234 is not present in the reconstruction tree because some of the housekeeping proteins were not available, impairing the

alignment. However, this bacterium was included in the comparison because it contains most of the genes analyzed in this study. R. palustris BisA53 was selected in preference to Nitrobacter Nb-31 1A because triclocarban it is phylogenetically closely related to B. japonicum. Mesorhizobium BNC1 (an EDTA-degrading bacterium formerly known as Agrobacterium sp. BNC1), Aurantimonas SI85-9A1 (a marine bacterium known by its role in Mn(II) oxidation, and unusual in its feature of possessing both the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase – RubisCO) and X. autotrophicus Py2 (a nitrogen-fixing methylotrophic, found in organic-rich soil, sediment, and water, and possessing genes responsible for alkene degradation) were selected by their proximity to the symbiotic bacteria in the phylogeny model (Figure 1), although they are not symbionts.

Apart from the 15-bp gap sequence, the PCR product has the same s

Apart from the 15-bp gap sequence, the PCR product has the same sequence as the wild-type VC1345 gene of 95-4. The PCR fragment was then cloned into the NcoI enzyme site of the expression vector pET15b (No. 69661-3; Novagen, Germany) and transformed into wild-type strain 95-4. The original VC1345 gene of 95-4 was also amplified and cloned into pET15b, then transformed into 95-4 as a control. Figure 1 The aligning maps of the sequences of VC1345 gene and the schematic diagram of the primers used in the function analysis of the 15bp gap of the VC1345 gene of

the O139 pigment producing V. cholerae strains. A. Mutation of the strain 3182 compared to other strains. B. Mutation of the O139 pigment producing strains. Two dashed boxes up the VC1345 gene sequence showed the short direct repeat at the deletion breakpoint. 2.4 Ribotyping Chromosomal DNAs of the test strains were extracted and VX-809 supplier digested with the enzyme BglI. DNA fragments were separated and transferred to nylon membranes. The membranes were prehybridized at 42°C for 2 h in hybridization solution without probe (2× SSC, 1% block reagent, 0.1% this website N-lauryl sarcosine, 0.02% SDS, and 50% formamide) and then hybridized with the freshly denatured labeled

gene probes at 42°C for 12 h. Hybridized membranes were washed twice in 2× SSC-0.1% SDS for 5 min at room temperature, followed by two washes in 0.1× SSC-0.1% SDS for 15 min at 68°C. The probe used in this typing was the PCR product of the conserved 16S rRNA gene of Escherichia coli, which was amplified by primers 5′-TTT

AAT GAC CAG CAC AGT-3′ and 5′-TCT GCC AGT GTT ACA ACC-3′, and was CH5183284 in vivo labeled using a random primer DIG DNA Labeling and Detection Kit (Roche Molecular Biochemicals, Indianapolis, IN). Detection was based on digoxigenin-anti digoxigenin ELISA, according to the manufacturer’s instructions. 2.5 Pulsed-field gel electrophoresis (PFGE) The PFGE protocol used was based on the PulseNet 1-day standardized PFGE protocol for V. cholerae [25]. The cell suspension in a polystyrene tube (Falcon; 12 by 75 mm) was adjusted to an optical density Teicoplanin of 4.0-4.2 using bioMerieux DENSIMAT; V. cholerae slices were digested with 20 U per slice NotI (New England Biolabs) for 4 h at 37°C. Electrophoresis was performed using a CHEF-DRIII system (Bio-Rad Laboratories). Images were captured using a Gel Doc 2000 system (Bio-Rad) and converted to TIFF files for computer analysis. The BioNumerics software package (version 4.0; Applied Maths, Inc.) was used to analyze the PFGE patterns. Fragments smaller than 20.5 kbp were not taken into account. Similarity analysis was performed by calculating Dice coefficients (SD), with customized tolerance for each EP. SD was calculated as follows: where n xy is the number of bands common to isolates x and y, n x is the total number of bands for isolate x, and n y is the total number of bands for isolate y.

Nature 450:575–578 doi:10 ​1038/​nature06262 PubMedCrossRef Spen

Nature 450:575–578. doi:10.​1038/​nature06262 PubMedCrossRef Spencer D, Wildman SG (1962) Observations on structure of grana-containing chloroplast

and a proposed model of chloroplast structure. Aust J Biol Sci 15:599–610 van Amerongen H, van Grondelle R (2001) Understanding the energy transfer function of LHCII, the major light-harvesting complex of green plants. J Phys Chem B 105:604–617. doi:10.​1021/​jp0028406 CrossRef van Oort B, Amunts A, Borst JW, van Hoek A, Nelson N, van Amerongen H (2008) Picosecond fluorescence of intact and dissolved PSI-LHCI crystals. Biophys J 95:5851–5861. doi:10.​1529/​biophysj.​108.​140467 PubMedCrossRef van Oort B, Murali S, Wientjes E, Koehorst RBM, Spruijt RB, van Hoek A, Croce R, van Amerongen H (2009) Ultrafast resonance energy transfer from a Dibutyryl-cAMP datasheet site-specifically attached fluorescent chromophore reveals the folding of the N-terminal domain of CP29. Chem Phys 357:113–119. doi:10.​1016/​j.​chemphys.​2008.​10.​052 CrossRef van Spronsen EA, Sarafis V, Brakenhoff GJ, van der Voort HTM, Nanninga N (1989) Three-dimensional structure of living chloroplasts as visualized by confocal scanning laser microscopy. Protoplasma 148:8–14. Acadesine doi:10.​1007/​BF01403986 CrossRef Visser HM, Kleima FJ, van Stokkum IHM, van Grondelle R, van Amerongen H (1996) Probing the many energy-transfer

processes in the photosynthetic light-harvesting complex II at 77 K using energy-selective sub-picosecond transient absorption spectroscopy. Chem Phys 210:297–312. doi:10.​1016/​0301-0104(96)00092-4 CrossRef Walla PJ, Yom J, Krueger BP, Fleming GR (2000) Two-photon excitation spectrum of light-harvesting complex II and fluorescence upconversion after one- and two-photon excitation of the carotenoids. J Phys Chem B 104:4799–4806. doi:10.​1021/​jp9943023 CrossRef Williams RM, Zipfel WR, Webb WW (2001) Multiphoton microscopy Alanine-glyoxylate transaminase in biological

research. Curr Opin Chem Biol 5:603–608. doi:10.​1016/​S1367-5931(00)00241-6 PubMedCrossRef Xu C, Zipfel W, Shear JB, Williams RM, Webb WW (1996) Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy. Proc Natl Acad Sci USA 93:10763–10768. doi:10.​1073/​pnas.​93.​20.​10763 PubMedCrossRef Zipfel WR, Williams RM, Webb WW (2003) Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol 21:1369–1377. doi:10.​1038/​nbt899 PubMedCrossRef Zucchelli G, Jennings RC, Garlaschi FM (1992) Independent fluorescence emission of the chlorophyll spectral forms in higher plant photosystem II. Biochim Biophys Acta 1099:163–169CrossRef”
“The conference An selleck chemicals llc International conference “Photosynthesis in the Global perspective” was held in Indore, India, during November 27–29, 2008, in honor of Professor Govindjee.

Similar to Karlsson, our lab has observed increased rpS6 phosphor

Similar to Karlsson, our lab has observed increased rpS6 phosphorylation 45 minutes after cycling exercise after both placebo and carbohydrate-protein beverages, although rpS6 phosphorylation was significantly higher after carbohydrate-protein compared to the placebo beverage [47]. Our lab has also observed timing of rpS6 phosphorylation in rats that was highly correlated to insulin [15]. rpS6 phosphorylation was higher 30 minutes post exercise in GSK923295 mw animals given carbohydrate-protein post exercise compared to

fasted, exercised controls. Interestingly, rpS6 phosphorylation was significantly increased at 90 minutes in animals that did not receive supplementation. At both time points, insulin was elevated in the respective animal groups compared to exercised controls. In the current study, we would expect the higher insulin and mTOR phosphorylation at 60 minutes after Cereal to

result in higher rpS6 phosphorylation compared to Drink, but that did not occur, possibly due to the amount of see more supplementation provided or biopsy timing. The nearly identical increase in rpS6 phosphorylation for both Cereal and Drink suggest that these changes were due to exercise and independent of supplementation. www.selleckchem.com/products/nutlin-3a.html For translation initiation to occur, mTOR must increase phosphorylation of eukaryotic translation initiation factor 4E (eIF4E) binding protein 1 (4E-BP1), releasing eIF4E to bind to eIF4G, forming the eIF4F complex. Phosphorylation of eIF4E may be affected by phosphorylation of MAP kinase interacting serine/threonine kinase 1 and 2 (MNK1/MNK2) [52]. Ueda et al. [52] established that changes in p38 MAPK phosphorylation of MNK1 directly influenced the levels of eIF4E phosphorylation while ERK1/2 activates both MNK1 and MNK2, but primarily affects the basal level of 5-Fluoracil eIF4E phosphorylation. The role of phosphorylated eIF4E in protein synthesis is unclear; while some studies have concluded that

phosphorylation of eIF4E is necessary for translation [53] others have not [52, 54, 55]. We observed a slight, insignificant decrease in phosphorylation of eIF4E after both Drink and Cereal, with no difference between treatments (Figure 6). This lack of change in phosphorylation of eIF4E between treatments agrees with the findings of Gautsch et al. [31], who observed no change in post-exercised rats that consumed saline, carbohydrate or a mixed meal. In addition, there was no difference in phosphorylation of eIF4E between fasted-rested rats and all exercise groups, suggesting that exercise did not affect eIF4E phosphorylation. The form of our recovery foods did not seem to affect our results, although the rate of gastric emptying would be expected to be lower for solid food versus liquid food. Reed et al.

First, ever since decolonisation, Asian governments have viewed t

First, ever since decolonisation, Asian governments have viewed the RG-7388 ic50 customary laws of their populations with mixed feelings (Antons 2003). They symbolise a link to ancient traditions

and are important symbols for national identity, but they are also suspect because of their potential to harbour pre-modern, sectarian and even secessionist tendencies. The constitutional provisions quoted above clearly show that in most cases, the rules of customary law are subordinated and made subject to the overriding imperatives of national development policies (Antons 2009b, p. 50). Secondly, it has been pointed out that colonisation, state building and globalisation have OSI-906 mouse affected customary “traditions” in many parts of the world to such an extent that they have to be rebuilt and become discursive weapons in negotiation processes rather than statements about the regularity of past practices (Chanock 2009; Zerner 1994). Chanock (2005) sees some prospects for combining what he calls “new custom” and contracts, but fears that radically divergent interests of resource users will make such compromises difficult. Traditional knowledge and access to biodiversity: The example of Indonesia Indonesia provides an example https://www.selleckchem.com/products/pf-03084014-pf-3084014.html of how many of these complex issues play out at the national level. The Indonesian government has recently been

involved in various disputes with Malaysia over cultural heritage and traditional cultural expressions in the form of songs,

Etofibrate handicrafts and dances (Antons 2009c; Gelling 2009). Traditional knowledge related to biodiversity, agriculture and traditional medicine has equally been the subject of cross-border disputes and “biopiracy” claims. Widely reported in the media (Antons and Antons-Sutanto 2009, pp. 382–383) were the patenting of Eurycoma longifolia, widely used in traditional medicine and known in Malaysia as Tongkat Ali and in Indonesia as Pasak Bumi (GRAIN and Kalpavriksh 2006); aborted attempts by a Japanese cosmetics manufacturer to patent compounds of traditional Indonesian medicinal plants (GRAIN 2008); the prosecution of a farmer from East Java under Law No. 12 of 1992 on Plant Cultivation Systems for selling non-certified seeds to neighbours (Jhamtani and Patria 2006); and longstanding claims about the patenting in the US of a traditional Indonesian formula for making a special type of soya bean cake (tempe) (Sardjono 2006, pp. 204–205). As in many other Asian developing countries, the role of the national government of Indonesia in the conservation and exploitation of natural resources remains strong. This strong position is enshrined in Article 33(3) of the Constitution, which provides that “the land, the waters and the natural resources within are controlled by the State and shall be used for the greatest possible welfare of the people.” It comes further to expression in two laws enacted by the Suharto government during the 1990s, Law No.

1%]) For patients treated with

1%]). For patients click here treated with intravenous therapy in the open-label

population, all ADRs occurred in <10 patients in both treatment groups at low incidence rates, i.e. nausea (moxifloxacin 5 [1.4%] versus comparator 2 [0.6%]), dizziness (moxifloxacin 6 [1.7%] versus comparator 6 [1.7%]), increased ALT (moxifloxacin 9 [2.6%] versus comparator 8 [2.3%]), and rash (moxifloxacin 8 [2.3%] versus comparator 3 [0.9%]). Table IV Adverse drug reactions occurring in either treatment group in ≧0.5% of patients valid for the safety analysis, treated with moxifloxacin or a comparator and stratified by route of administration (oral only; intravenous followed by oral [sequential]; intravenous only) and by study design (double blind, open label). Numbers in bold italic text correspond to events with an incidence ≥5% in either treatment group. A single asterisk this website (*) indicates differences observed between groups that were ≥2.5% for events with an incidence ≥2.5% in both groups or ≥2-fold for events with an incidence <2.5% in one or both groups (calculations were made using the number of patients [no rounding]; GS-9973 solubility dmso in the event of a null value for one treatment, only situations where ≥2 cases were observed in the other treatment group are indicated); the symbol is placed to the right of the value observed

for the drug in disfavor. A double asterisk (**) indicates differences observed between treatment groups according to the same rule and where the number of patients experiencing an event was ≥10 (-)-p-Bromotetramisole Oxalate in either group; the symbols are placed to the right of the value observed for the drug in disfavor Serious AEs and Serious ADRs Treatment-emergent SAEs are presented by SOCs for combined double-blind and open-label studies in table V. In the oral population, the overall incidence of SAEs (4.0% versus

3.9% in moxifloxacin- and comparator-treated patients) and those within the SOCs were very similar in the treatment groups. More SAEs were reported in the intravenous/oral studies in both treatment groups (moxifloxacin 595 [17.3%] versus comparator 527 [15.4%]), as expected given the increased severity of the disease. The SOCs associated with the highest incidences of events in both treatment groups, were ‘infections and infestations’ (moxifloxacin 219 [6.4%] versus comparator 165 [4.8%]) and ‘respiratory, thoracic, and mediastinal disorders’ (moxifloxacin 129 [3.8%] versus comparator 143 [4.2%]). Serious ‘cardiac disorders’ in the population treated by the intravenous/oral routes were reported with a similar incidence in the two groups (moxifloxacin 84 [2.4%] versus comparator 89 [2.6%]). In the intravenous-only trials, the overall rates were 7.9% and 6.0% in moxifloxacin- and comparator-treated patients, respectively, with SAEs from the SOC ‘infections and infestations’ being predominant (moxifloxacin 38 [4.1%] versus comparator 23 [2.5%]).

This situation is seen particularly clearly with thicker TiO2 lay

This situation is seen particularly clearly with thicker TiO2 layers. To evaluate this spectral shift, one should solve the electromagnetic problem describing the geometry

presented in insets a-c in Figure 9. JQ-EZ-05 nmr However, there still is no any exact solution for this problem, and the reported numerical calculations [27] performed for an isolated hemisphere in a uniform dielectric surrounding (ϵ sub = ϵ cover) have shown that even in this case about 1% rounding of the hemisphere edge results in a meaningful shift of the resonant frequency. In measurements, it is difficult to characterize the curvature of the edges of a nanoisland formed in SOD on a glass substrate, and this does not allow constructing a numerical model for this situation. We can only assume that the shapes of the nanoislands in differently prepared MIFs are very similar. This is indeed indicated by the inset in GSK1210151A supplier Figure 5 as the shift of the SPR under the thickest TiO2 cover is practically the same for all the samples. Figure 9 Schematic of SPR electric field localization (lateral component) in MIF for different dielectric

cover thicknesses. https://www.selleckchem.com/products/pnd-1186-vs-4718.html The spectral shift of the SPR saturates when the electric field E generated by a nanoisland under probing electromagnetic wave is completely localized within the covering film and the glass substrate as shown in Figure 9 (inset c). For thinner TiO2 films, the tail of the SPR electric field penetrates through the covering layer, that is,

the electric field is partly localized in the air (see Figure 9, inset b). In other words, the effective dielectric permittivity of the nanoisland surrounding is less for thinner covers than for thicker covers. This results in weaker dielectric loading of the SPR and corresponds to its unsaturated spectral shift, which tends to saturate with the TiO2 film thickness increase. Thus, the saturated SPR shift indicates that the thickness of the cover exceeds the length of the SPR electric field penetration into the cover (Figure 9, inset c). As measured with absorption spectroscopy, the spectral shift of the SPR in TiO2-covered MIF saturates at about 40- to 50-nm cover Ribonucleotide reductase thickness. We can suppose that the SPR electric field intensity decays in TiO2 film at about the same length. Unfortunately, comparing the dependences of the SPR spectral shift in Figure 5, one can hardly conclude whether there is a difference in the SPR decay length for differently prepared MIFs. The measured Raman scattering signal I Raman should decay much faster. If the glass surface is covered with silver nanospheres, [28] for separate molecules and [29] for a monolayer of an analyte, where r is the radius of silver microsphere and d is the distance from the microsphere to the analyte.

Primer3 software

Primer3 software LXH254 nmr was used to design discriminating PCR primers based on the set of discriminating locations identified. Three primers were designed at each discriminating

location: a 5′-forward primer with the node X call in the 3′ position; a 5′-forward primer with the node Y call in the 3′ position; and a single 3′-reverse primer. A base call at the discriminating location is determined by two PCR reactions where one of the two yields a lower cycle threshold (Ct) value. The RT-PCR primers used are shown in Additional File 2. Real-time PCR assays for F. tularensis typing Real-time PCR assays to identify F. tularensis subspecies and clades were developed using SYBR® Green (BioRad, Hercules CA) which binds all dsDNA molecules, emitting a fluorescent signal of a defined wavelength (522 nm). Reactions were performed in 20 μl volume and contained 80 pg of genomic DNA (0.01 ng/μl), 150 nM of forward and reverse primers and 10 μl of iQ SYBR® Green Supermix (BioRad, Hercules CA). Reaction components were mixed in a Selleck G418 V-bottom thin wall PCR 96-well plate (BioRad, Hercules CA). Real-time PCR was performed

Selleck AICAR using the iCycler iQ (BioRad, Hercules, CA) with the following thermal cycling parameters: 50°C for 2 min, 95°C for 5 min, 60 cycles of 95°C for 15 seconds and 68°C for 30 seconds, 72°C for 30 seconds, 95°C for 1 min and finally 55°C for 3 min. The fluorescence was measured at 72°C in the cycle program. A cycle threshold (Ct) was automatically generated by the iCycler iQ Version 3.0a analysis software for each amplification reaction (BioRad, Hercules CA).

Melt curve analysis was performed to verify that no primer dimers formed. Results Whole genome resequencing of strains Previously, we reported an Affymetrix Inc. GeneChip® array based whole genome resequencing platform for F. tularensis. Our whole-genome sequencing by hybridization approach made use of a set of bioinformatic filters to eliminate a majority of false positives and indicated a base call accuracy of 99.999% (Phred equivalent score 50) for type B strain LVS [13]. The base call accuracy was determined by comparing the base calls remaining after the application of our filters to the published sequence Buspirone HCl of the LVS strain. The bioinformatic filter programs may be accessed at http://​pfgrc.​jcvi.​org/​index.​php/​compare_​genomics/​snp_​scripts.​html. Two type A strains, WY96 3418 and SCHU S4 showed base call accuracies of 99.995% and 99.992% with Phred equivalent scores of 43 and 41 respectively [13]. We used this approach to collect whole-genome sequence and global SNP information from 40 Francisella strains. Table 1 shows the list of strains analyzed in this study. Twenty six type A (20 A1 and 6 A2), thirteen type B and one F. novicida strain were resequenced. The base call rate and number of SNPs for F. tularensis A1, A2 and type B strains are shown in Figure 1 and Additional File 3.

2009) An example from zoology is the study by Zuluaga-Montero et

2009). An example from zoology is the study by Zuluaga-Montero et al. (2010), focusing on sea fans (Gorgonia ventalina), in which the results indicated Vactosertib cell line that the fungal community composition did

not differ significantly between healthy and diseased tissues in each reef and that the differences in fungal communities were more attributable to differences between reefs than to the health of the studied colonies. Defining a fungus as a pathogen implies a difference in its incidence and certainly in its PF-02341066 clinical trial abundance between healthy and diseased individuals. The appearance of the disease symptoms should be the consequence of the increasing proliferation of the causal pathogen and this should have an impact on the fungal community structure. In the case of esca, such a shift in fungal community structure is not observed. In our study, however, a single fungal OTU (based on ITS similarity) possibly embraces very closely related species, subspecies or strains that have a different virulence

and could be differentially associated with healthy or diseased plants, as for instance in the case of Alternaria (Table 1, Pryor and Michailides 2002), Phaeomoniella chlamydospora (Mostert et al. 2006) or Phaeoacremonium angustius (Santos et al. 2005). SYN-117 purchase Also, cumulated small differences in abundance of several OTUs might eventually differentiate between healthy and diseased plants, but such slight differences in abundance are, each taken separately, too small to contribute to a significant distinction between healthy and diseased plants in a PCA analysis (Fig. 6). Nevertheless, our experiment was conducted

in a single, small vineyard plot, making it unlikely to observe differences in virulence between strains or subspecies associated with adjacent plants. If some strains were indeed more virulent within a single OTU, this would have resulted in an increase of the abundance of such an OTU in diseased grapevine plants, as a more virulent strain is expected to be more invasive than less virulent ones. Neither is it likely that unculturable fungi are responsible for the emergence of esca in the sense that a shift toward pathogenicity – and consequently invasiveness – of these fungi Rebamipide should also have an impact on the culturable part of the fungal community associated with grapevine, which is not the case in our study. Nevertheless, there remains an urgent need to characterize the genotypes of the fungi associated with esca disease in more detail before we can firmly exclude fungi as the principal cause of esca. Other organisms, such as bacteria, may be involved in esca but eventual differences between the bacterial communities associated with diseased or healthy grapevines have never been studied. As suggested by Bertsch et al.

The use of sports supplements by gymnastics athletes is very rare

The use of sports supplements by gymnastics athletes is very rare, being caloric restriction the main nutritional strategy for this population. Carbohydrates supplements might be useful, since is well established as an ergogenic resource [11], being considered an essential energy supply for high intensity exercise [12] an immediate energy source either to the muscle tissue or to the nervous system, Entospletinib nmr as a critical fuel for neurons [13], delaying fatigue that might be seen as an interruption of the information traffic from the brain to the muscle [14].

Therefore, the aim of this study was to investigate the influence of fatigue on see more the artistic gymnastic athlete performance and the influence of carbohydrate supplementation on their performance and fatigue. Methods Sample and ethical aspects 15 female artistic gymnastic athletes, from 11 to 14 years old, took part in the study. All of them were healthy and had a high training level, at least 5 times a week, 4 hours a day. Athletes were selected from the kids Barueri training team and they had at least 2 years of experience. The study design was submitted to the Ethical Committee of Mackenzie Presbyterian University, and was in accordance with the Helsinki Declaration (1975). After the approval (under

the protocol number many CAAE 0032.0.272.000-10), because the subjects were under 18, we set up a meeting with the athletes coach and their parents, so they could be informed of the study procedures and sign an informed consent form if they agreed with the study. During the study, subjects were taught to leave the study protocol if they wanted or felt any discomfort. Experimental procedures Athletes were divided randomly in two groups, control group (CG), and the previously submitted to fatigue group (FG). On the first day (WATER DAY) CG did a previous warm up of 10 minutes followed by 5 sets of determined

exercises (Hanging straight leg raise, scale, gymnastic turns, handstands, cartwheel, Split Leaps, walkover, a dismount with front flip) on the balance beam. FG did a fatigue circuit of 20 minutes, a 10 minutes specific warm up and then the 5 sets of the same exercises of CG. The fatigue circuit consisted of 3 sets of 10 exercises usually performed by artistic gymnastic athletes. The protocol was very intense; the athletes reported that it was close to 90% of the rate of perceived exertion. Exercises familiar to the athletes were chosen and their coach this website helped to keep the athletes performing them at high intensity up to the end of the 20 minutes. The objective of the fatigue circuit was to simulate a competition day, where the balance beam is the last apparatus to be performed.